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Microbial Transformation of (+)-α-Pinene to (+)-Verbenone by Resting Cell Suspension of Cladosporium Cladosporides

[ Vol. 1 , Issue. 2 ]

Author(s):

Siddhardha Busi, Ramanjaneyulu GS and Prabhakar S   Pages 129 - 131 ( 3 )

Abstract:


Biotransformation of (+)-α-pinene to (+)-verbenone using shaking cultures of Cladosporium cladosporides (MTCC 1003) was investigated. Cladosporium cladosporides showed specific transformation of (+)-α-pinene with 86.8±3.0% conversion (relative integrated area gas chromatography–mass spectrometry (GC-MS)) of (+)-verbenone in 7 day incubation. The amount of verbenone formed was observed at 1, 3, 5 and 7th day where it is gradually increased. Verbenol was formed as intermediate product and slowly biocatalyzed to verbenone.

Keywords:

Biotransformation, Cladosporium cladosporides, Verbenone, Oxidation, GC-MS

Affiliation:

Microbiology Programme, Department of Biochemistry and Molecular Biology, Pondicherry University, Puducherry – 605014, India.



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