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Immobilization of Amano AK lipase from Pseudomonas fluorescens on novel silk microfiber using Oxone®: Parameter optimization for enzymatic assays and use in esterification of residual palm oil

Author(s):

Evaldo B. M. Júnior, Fernando B. Neves, Samuel Q. Lopes , Fabrício H. Holanda , Tiago M. Souza , Erveton P. Pinto, Alex N. Oliveira, Luis P. Fonseca, Sérgio A. Yoshioka and Irlon M. Ferreira*  

Abstract:


Background: Biodiesel has been shown to be effectively produced by immobilized enzymatic catalysts. The selection of support material is a prominent factor for obtaining an efficient lipase. Silk fibroin (SF) is a natural polymer, produced by glands of some arthropods, especially by the Bombyx mor, attracting attention for immobilization lipase attention.

Objective: This paper presents a novel method to obtain silk microfibers (SMF) from Oxone® salt in water, used as support for Amano AK lipase from Pseudomonas fluorescens in biodiesel production from deodorization distillate of palm oil (DDPO).

Method: The oxone® salt in the presence of Ca2+ ions act as a mineralizing agent in the peptide bonds present in silk fibroin, altering some of its physical and chemical properties, such as zeta potential, crystallinity, micro-morphology, infrared spectroscopic profile, and showing formation or absence of SF original connections.

Results: The modified support was tested as a support alternative for the immobilization of Amano AK lipase from Pseudomonas fluorescens. Enzyme activity values indicated that lipase immobilization on SMF was efficient as a heterogeneous catalyst in the esterification of DDPO (deodorization distillate palm oil).

Conclusion: The effect of some reaction parameters, such as catalyst concentration, molar ratio, temperature, and reaction time, was studied to optimize the conditions for maximum conversion of DDPO (40.5%).

Keywords:

Pseudomonas fluorescens, microfibrils, silk fibroin, Oxone®, deodorization distillate palm oil.

Affiliation:

Laboratório de Biocatálise e Síntese Orgânica Aplicada, Universidade Federal do Amapá, Rod. Juscelino Kubitschek, Km 02, Jardim Marco Zero, 68902-280, Macapá, AP, Laboratório de Biocatálise e Síntese Orgânica Aplicada, Universidade Federal do Amapá, Rod. Juscelino Kubitschek, Km 02, Jardim Marco Zero, 68902-280, Macapá, AP, Laboratório de Biocatálise e Síntese Orgânica Aplicada, Universidade Federal do Amapá, Rod. Juscelino Kubitschek, Km 02, Jardim Marco Zero, 68902-280, Macapá, AP, Laboratório de Biocatálise e Síntese Orgânica Aplicada, Universidade Federal do Amapá, Rod. Juscelino Kubitschek, Km 02, Jardim Marco Zero, 68902-280, Macapá, AP, Departamento de Engenharia Química, Universidade do Estado do Amapá, 68900-070 Macapá, AP, Departamento de Ciências Exatas e Tecnológicas, Universidade Federal do Amapá, Rod. Juscelino Kubitschek, Km 02, Jardim Marco Zero, 68902-280, Macapá, AP, Laboratório de Biocatálise e Síntese Orgânica Aplicada, Universidade Federal do Amapá, Rod. Juscelino Kubitschek, Km 02, Jardim Marco Zero, 68902-280, Macapá, AP, Bioengineering Department, Instituto Superior Técnico, University of Lisbon, Institute for Bioengineering and Biosciences, Av. Rovisco Pais, 1049-001 Lisbon, Instituto de Química de São Carlos, Universidade de São Paulo, Av. Trabalhador São-Carlense, 400, J. Arnold Schimidt, 13563-590, São Carlos, SP, Laboratório de Biocatálise e Síntese Orgânica Aplicada, Universidade Federal do Amapá, Rod. Juscelino Kubitschek, Km 02, Jardim Marco Zero, 68902-280, Macapá, AP



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